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*indicates equal contributions, # corresponding author(s)
Underlined link to DOI or Pubmed ID.



Under development 



Bayne AN, Dong J, Amiri S, Farhan SMK#, Trempe J-F# (2021) MTSviewer: a database to visualize mitochondrial targeting sequences, cleavage sites, and mutations on protein structures. BioRxiv, DOI:

Duchesne A*, Dong J*, Bayne AN, Mohamed N-V, Mathur M, Fon EA, Durcan TM, Trempe J-F# (2021) An Approach to Measuring Protein Turnover in Human Induced Pluripotent Stem Cell Organoids by Mass Spectrometry. BioRxiv, DOI:


Vranas M*, Lu Y*, Rasool S, Croteau N, Krett JD, Sauvé V, Gehring K, Fon EA, Durcan TM, Trempe J-F# (2021) Selective localization of Mfn2 near PINK1 enable its preferential ubiquitination by Parkin on mitochondria. BioRxiv, DOI:


Rasool S, Veyron S, Soya N, Eldeeb M, Lukacs GL, Fon EA, Trempe J-F# (2021) Mechanism of PINK1 activation by autophosphorylation and insights into assembly on the TOM complex. Molecular CellPMID 34875213


Shi F, Aloufi N, Traboulsi H, Trempe JF, Eidelman DH, Baglole CJ# (2021) Endogenous regulation of the Akt pathway by the aryl hydrocarbon receptor (AhR) in lung fibroblasts. Scientific Reports, 11(1):23189. PMID 34848742


Sosero YL, Yu E, Estiar MA, Krohn L, Mufti K, Rudakou U, Ruskey JA, Asayesh F, Laurent SB, Spiegelman D, Trempe JF, Quinnell TG, Oscroft N, Arnulf I, Montplaisir JY, Gagnon JF, Desautels A, Dauvilliers Y, Gigli GL, Valente M, Janes F, Bernardini A, Sonka K, Kemlink D, Oertel W, Janzen A, Plazzi G, Antelmi E, Biscarini F, Figorilli M, Puligheddu M, Mollenhauer B, Trenkwalder C, Sixel-Döring F, Cochen De Cock V, Monaca CC, Heidbreder A, Ferini-Strambi L, Dijkstra F, Viaene M, Abril B, Boeve BF, Postuma RB, Rouleau GA, Ibrahim A, Stefani A, Högl B, Hu MTM, Gan-Or Z# (2021) Rare PSAP Variants and Possible Interaction with GBA in REM Sleep Behavior Disorder. J Parkinson Disease. PMID 34690151


Torrealba-Acosta G#, Yu E, Lobo-Prada T, Ruíz-Martínez J, Gorostidi-Pagola A, Gan-Or Z, Carazo-Céspedes K, Trempe JF, Mata IF, Fornaguera-Trías J (2021) Clinical and Genetic Analysis of Costa Rican Patients With Parkinson's Disease. Frontiers Neurology,12: 656342. PMID 34421783


Varghaei P, Yoon G, Estiar MA, Veyron S, Leveille E, Dupré N, Trempe JF, Rouleau GA, Gan-Or Z# (2021) GCH1 mutations in hereditary spastic paraplegia. Clinical Genetics, 100:51-58. PMID 33713342


Estiar MA, Yu E, Haj Salem I, Ross JP, Mufti K, Akçimen F, Leveille E, Spiegelman D, Ruskey JA, Asayesh F, Dagher A, Yoon G, Tarnopolsky M, Boycott KM, Dupre N, Dion PA, Suchowersky O, Trempe JF, Rouleau GA, Gan-Or Z# (2021) Evidence for Non-Mendelian Inheritance in Spastic Paraplegia 7. Movement Disorders, 36:1664-1675. PMID 33598982


Khan N*, Pelletier D*, McAlear TS, Croteau N, Veyron S, Bayne AN, Black C, Ichikawa M, Khalifa AAZ, Chaaban S, Kurinov I, Brouhard G, Bechstedt S, Bui KH, Trempe J-F# (2021) Crystal structure of human PACRG in complex with MEIG1 reveals roles in axoneme formation and tubulin binding. Structure, 29:572-586. PMID 33529594


Mufti K, Yu E, Rudakou U, Krohn L, Ruskey JA, Asayesh F, Laurent SB, Spiegelman D, Arnulf I, Hu MTM, Montplaisir JY, Gagnon JF, Desautels A, Dauvilliers Y, Gigli GL, Valente M, Janes F, Bernardini A, Högl B, Stefani A, Holzknecht E, Sonka K, Kemlink D, Oertel W, Janzen A, Plazzi G, Antelmi E, Figorilli M, Puligheddu M, Mollenhauer B, Trenkwalder C, Sixel-Döring F, Cochen De Cock V, Monaca CC, Heidbreder A, Ferini-Strambi L, Dijkstra F, Viaene M, Abril B, Boeve BF, Trempe J-F, Rouleau GA, Postuma RB, Gan-Or Z# (2021). Novel Associations of BST1 and LAMP3 With REM Sleep Behavior Disorder. Neurology. e1402-e1412. PMID 33397775


Robert RF, Bayne AN, Goiran T, Lévesque D, Boisvert F-M, Trempe J-F, Fon EA# (2021) Proteomic profiling of mitochondrial-derived vesicles in brain reveals enrichment of respiratory complex sub-assemblies and small TIM chaperones. Journal of Proteome Research, 20: 506-517. PMID 33242952



Eldeeb MA, Bayne AN, Trempe J-F, Fon EA# (2020) Fine-tuning TOM-mitochondrial import via ubiquitin. Trends in Cell Biology, 30: 425-427. PMID 32345464


Khalifa A, Ichikawa M, Dai D, Black C, Peri K, McAlear TS, Kubo S, Veyron S, Yang SK, Vargas J, Trempe J-F, Bechstedt S, Bui KH# (2020) The inner junction complex of the cilia is an interaction hub that involves tubulin post-translational modifications. Elife ,e52760, PMID 31951202


Semmler S, Gagné M, Garg P, Pickles SR, Baudouin C, Hamon-Keromen E, Destroismaisons L, Khalfallah Y, Chaineau M, Caron E, Bayne AN, Trempe J-F, Cashman NR, Star AT, Haqqani AS, Durcan TM, Meiering EM, Robertson J, Grandvaux N, Plotkin SS, McBride HM,  Vande Velde C# (2020) The E3 ubiquitin ligase TRAF6 is a novel interacting protein of amyotrophic lateral sclerosis-linked misfolded SOD1. Journal of Biological Chemistry, 295: 3808-3825, PMID 32029478


Estiar MA, Léveillé E, Spiegelman D, Dupré N, Trempe J-F, Rouleau GA, Gan-Or Z# (2020) Clinical and genetic analysis of ATP13A2 in hereditary spastic paraplegia expands the phenotype. Molecular Genetics Genomic Medecine, pii: e1052. PMID 31944623


Krohn L, Öztürk TN, Vanderperre B, Ouled Amar Bencheikh B, Ruskey JA, Laurent SB, Spiegelman D, Postuma RB, Arnulf I, Hu MTM, Dauvilliers Y, Högl B, Stefani A, Monaca CC, Plazzi G, Antelmi E, Ferini-Strambi L, Heidbreder A, Rudakou U, Cochen De Cock V, Young P, Wolf P, Oliva P, Zhang XK, Greenbaum L, Liong C, Gagnon JF, Desautels A, Hassin-Baer S, Montplaisir JY, Dupré N, Rouleau GA, Fon EA, Trempe JF, Lamoureux G, Alcalay RN, Gan-Or Z# (2020) Genetic, Structural, and Functional Evidence Link TMEM175 to Synucleinopathies. Annals of Neurology, 87: 139-153. PMID 31658403




Bayne AN, Trempe J-F# (2019) Mechanisms of PINK1, ubiquitin and Parkin interactions in mitochondrial quality control and beyond. Cellular and Molecular Life Sciences, 76: 4589-4611. PMID 31254044


Léveillé E, Estiar MA, Krohn L, Spiegelman D, Dionne-Laporte A, Tarnopolsky M, Boycott KM, Yoon G, Suchowersky O, Dupré N, Trempe J-F, Rouleau GA, Gan-Or Z# (2019) SPTAN1 mutations cause autosomal recessive hereditary spastic paraplegia. Journal of Human Genetics, 64: 1145-1151. PMID 31515523


Fava VM, Xu YZ, Lettre G, Thuc NV, Orlova M, Thai VH, Croteau N, Eldeeb MA, MacDougall EJ, Cambri G, Tao S, Lahiri R, Adams L, Fon EA, Trempe J-F, Cobat A, Alcaïs A, Abel L, Schurr E# (2019) Pleiotropic effects for Parkin and LRRK2 in leprosy type-1 reactions and Parkinson’s Disease. Proceedings of the National Academy of Sciences U.S.A. 116: 15616-15624. PMID 31308240


Svoboda M, Konvalinka J, Trempe J-F, Šašková KG# (2019). The yeast proteases Ddi1 and Wss1 are both involved in the DNA replication stress response. DNA Repair (Amst), 80: 40-51. PMID 31276951


Yi W, MacDougall EJ, Tang MY, Krahn AI, Gan-Or Z, Trempe J-F, Fon EA# (2019) The Landscape of Parkin Variants Reveals Pathogenic Mechanisms and Therapeutic Targets in Parkinson's Disease. Human Molecular Genetics, pii: ddz080. PMID 30994895


Alcalay RN, Mallett V, Vanderperre B, Tavassoly O, Dauvilliers Y, Leblond CS, Ambalavanan A, Laurent SB, Spiegelman D, Dionne-Laporte A, Liong C, Levy OA, Fahn S, Waters C, Kuo SH, Chung WK, Ford B, Marder KS, Kang UJ, Hassin-Baer S, Greenbaum L, Trempe J-F, Wolf P, Oliva P, Zhang XK, Clark LN, Langlois M, Dion PA, Fon EA, Dupré N, Rouleau GA, Gan-Or Z (2019) SMPD1 mutations activity and a-synuclein accumulation in Parkinson's disease. Movement Disorders, 34: 526-535. PMID 30788890


Laughlin TG, Bayne AN, Trempe J-F, Savage DF, Davies KM#. (2019) Structure of NDH the complex I-like molecule of oxygenic photosynthesis. Nature, 566: 411-414. PMID 30742075




Trempe, J-F#, Gehring K# (2018). Small-angle X-ray scattering for the study of proteins in the ubiquitin pathway. In: Mayor & Kleiger (eds) The Ubiquitin Proteasome System, Methods in Molecular Biology. 1844: 197-208. PMID 30242711


Rasool S, Trempe J-F# (2018). New insights into the structure of PINK1 and the mechanism of ubiquitin phosphorylation. Critical Reviews in Biochemistry and Molecular Biology. 21: 1-20. PMID 30238821


Sauvé V*, Sung G*, Soya N, Kozlov G, Blaimschein N, Miotto LS, Trempe J-F#, Lukacs GL, Gehring K# (2018). Mechanism of parkin activation by phosphorylation. Nature Structural and Molecular Biology, 25: 623-630. PMID 29967542


Ménade M*, Kozlov G*, Trempe J-F*, Pande H, Shenker S, Wickremasinghe S, Dicaire M-J, Li X, Brais B, McPherson PS, Gehring K# (2018). Structures of Ubl and Hsp90-like domains of sacsin provide insight into pathological mutations. Journal of Biological Chemistry, 293: 12832-42. PMID 29945973


Ruskey JA, Zhou S, Santiago R, Franche LA, Alam A, Roncière L, Spiegelman D, Fon EA, Trempe J-F, Kalia LV, Postuma RB, Dupre N, Rivard GE, Assouline S, Amato D, Gan-Or Z# (2018). The GBA p.Trp378Gly mutation is a probable French-Canadian founder mutation causing Gaucher disease and synucleinopathies. Clinical Genetics, 94:339-345. PMID 29920646


McLelland G-L, Yi W, Dorval G, Chen CX, Lauinger ND, Valimehr S, Rakovic A, Rouiller I, Durcan TM, Trempe J-F, Fon EA# (2018). Mfn2 ubiquitination by PINK1/parkin gates the p97-dependent release of ER from mitochondria to drive mitophagy. eLife, 7:e32866. PMID 29676259


Rasool S, Soya N*, Truong L*, Croteau N, Lukacs G, Trempe J-F# (2018). PINK1 autophosphorylation is required for ubiquitin recognition. EMBO Reports, 19: e44981. doi: 10.15252/embr.201744981. PMID 29475881




Tang MY*, Vranas M*, Krahn AI, Pundlik S, Trempe J-F#, Fon EA# (2017) Structure-guided mutagenesis reveals a hierarchical mechanism of Parkin activation. Nature Communications, 8:14697. PMID 28276439



Trempe J-F#, Šašková KG, Sivá M, Ratcliffe CD, Veverka V, Hoegl A, Ménade M, Feng X, Shenker S, Svoboda M, Kožíšek M, Konvalinka J, Gehring K (2016). Structural studies of the yeast DNA damage-inducible protein Ddi1 reveal domain architecture of this eukaryotic protein family. Scientific Reports, 6:33671. PMID 27646017


Sivá M, Svoboda M, Veverka V, Trempe J-F, Hofmann K, Kožíšek M, Hexnerová R, Sedlák F, Belza J, Šácha P, Hubálek M, Starková J, Flaisigová I, Konvalinka J, Šašková KG# (2016). Human DNA-Damage-Inducible
2 Protein is structurally and functionally distinct from its yeast ortholog. Scientific Reports, 6:30443. PMID 27461074




Sauvé V*, Lilov A*, Seirafi M*, Vranas MM, Rasool S, Kozlov G, Sprules T, Wang J, Trempe J-F#, Gehring K#. (2015) A Ubl/ubiquitin switch in the activation of Parkin. EMBO Journal, e201592237. PMID 26254305


Aguileta MA, Korac J, Durcan TM, Trempe J-F, Haber M, Gehring K, Elsasser S, Waidmann O, Fon EA, Husnjak K. (2015) The E3 ubiquitin ligase parkin is recruited to the 26 S proteasome via the proteasomal ubiquitin receptor Rpn13. Journal of Biological Chemistry, 290: 7492-505. PMID 25666615



Koyano F, Okatsu K, Kosako H, Tamura Y, Go E, Kimura M, Kimura Y, Tsuchiya H, Yoshihara H, Hirokawa T, Endo T, Fon EA, Trempe J-F, Saeki Y, Tanaka K, Matsuda N. (2014) Ubiquitin is phosphorylated by PINK1 to activate parkin. Nature, 510: 162-6. PMID 24784582

2001-2013 (MSC, PHD, POSTDOC)

Trempe J-F*, Sauvé V*, Grenier K, Seirafi M, Tang MY, Ménade M, Al-Abdul-Wahid S, Krett J, Wong K, Kozlov G, Nagar B, Fon EA#, Gehring K# (2013) Structure of parkin reveals mechanisms for ubiquitin ligase activation. Science, 340: 1451-1455. PMID 23661642


Trempe J-F#, Fon EA# (2013) Structure and function of Parkin PINK1 and DJ-1 the three musketeers of neuroprotection. Frontiers in Neurology 4: 38. PMID 23626584

Grishin AM, Ajamian E, Tao L, Bostina M, Zhang L, Trempe J-F, Menard R, Rouiller I, Cygler M (2013) Family of phenylacetyl-CoA monooxygenases differs in subunit organization from other monooxygenases. Journal of Structural Biology, 184: 147-54. PMID 24055609


Trempe J-F#, Shenker S, Kozlov G, Gehring K (2011) Self-association studies of the bifunctional N-acetylglucosamine-1-phosphate uridyltransferase from Escherichia coli. Protein Science 20: 745-752. PMID 21370307


Trempe J-F# (2011) Reading the ubiquitin postal code. Current Opinion in Structural Biology 21: 792-801. PMID 22036065


Kozlov G, Bastos-Aristizabal S, Maattanen P, Rosenauer A, Zheng F, Killikelly A, Trempe J-F, Thomas DY, Gehring K (2010) Structural basis of cyclophilin B binding by the calnexin/calreticulin P-domain. Journal of Biological Chemistry 285: 35551-35557. PMID 20801878


Riedinger C*, Boehringer J*, Trempe J-F*, Lowe ED, Brown NR, Gehring K, Noble MEM, Gordon C, Endicott JA (2010) The structure of Rpn10 and its interactions with polyubiquitin chains and the proteasome subunit Rpn12. Journal of Biological Chemistry 285: 33992-34003. PMID 20739285


Trempe J-F#, Brown NR, Noble MEM, Endicott JA (2010) A new crystal form of Lys48-linked diubiquitin. Acta Crystallographica Section F 66: 994-998. PMID 20823512


Shi R, Proteau A, Villarroya M, Moukadiri I, Zhang L, Trempe J-F, Matte A, Armengod ME, Cygler M (2010) Structural Basis for Fe-S Cluster Assembly and tRNA Thiolation Mediated by IscS Protein-Protein Interactions. PLoS Biology 8: e1000354. PMID 20404999


Trempe J-F*, Chen CXQ*, Grenier K, Camacho EM, Kozlov G, McPherson PS, Gehring K, Fon EA (2009) SH3 domains from a subset of BAR-proteins define a novel Ubl-binding domain and implicate parkin in synaptic ubiquitination. Molecular Cell 36: 1034-1047. PMID 20064468


Matte A#, Kozlov G, Trempe J-F, Currie MA, Burk D, Jia Z, Gehring K, Ekiel I, Berghuis AM, Cygler M (2009) Preparation and characterization of bacterial protein complexes for structural analysis. Advances in Protein Chemistry and Structural Biology 76 : 1-42. PMID 20663477


Matta-Camacho E, Kozlov G, Trempe J-F, Gehring K (2009) Atypical binding of the Swa2p UBA domain to ubiquitin. Journal of Molecular Biology 386: 569-577. PMID 18948116


Trempe J-F, Pomerantseva E, Gehring K (2008) REDOR recoupling in polymer-stabilized liquid crystals undergoing MAS: two-dimensional NMR applications in strongly aligned proteins. Canadian Journal of Chemistry 86 : 608-615. doi : 10.1139/V08-048.


Trempe J-F, Endicott JA# (2007) Structural biology: Pass the protein. Nature 445 375-376. PMID 17220873


Lowe ED, Hasan N, Trempe J-F, Fonso L, Noble MEM, Endicott JA, Johnson LN, Brown NR (2006) Structures of the Dsk2 UBL and UBA domains and their complexes. Acta Crystallographica Section D 62: 177-188. PMID 16421449


Trempe J-F, Brown NR, Lowe ED, Gordon C, Campbell ID, Noble MEM, Endicott JA (2005) Mechanism of Lys48-linked polyubiquitin chain recognition by the Mud1 UBA domain. EMBO Journal 24 :3178-3189. PMID 16138082


Pollock S, Kozlov G, Pelletier MF, Trempe J-F, Jansen G, Sitnikov D, Bergeron JJ, Gehring K, Ekiel I, Thomas DY (2004) Specific interaction of ERp57 and calnexin determined by NMR spectroscopy and an ER two-hybrid system. EMBO Journal 23 : 1020-1029. PMID 14988724


Gutiérrez P, Osborne MJ, Siddiqui N, Trempe J-F, Arrowsmith C, Gehring K (2004) Structure of the archaeal translation initiation factor aIF2 beta from Methanobacterium thermoautotrophicum: implications for translation initiation. Protein Science 13: 659-667. PMID 14978306


Kozlov G, De Crescenzo G, Lim NS, Siddiqui N, Fantus D, Kahvejian A, Trempe  J-F, Elias D, Ekiel I, Sonenberg N, O’Connor-McCourt M, Gehring K (2004) Structural basis of ligand recognition by PABC, a highly specific peptide-binding domain found in poly(A)-binding protein and a HECT ubiquitin ligase. EMBO Journal, 23 : 272-281. PMID 14685257


Trempe J-F, Gehring K# (2003) Observation and interpretation of residual dipolar couplings in biomolecules, in NMR of Ordered Liquids (eds. E. Elliott Burnell and Cornelius A. de Lange) Chapter 8, pp. 163-190, Kluwer Academic Publishers, The Netherlands. 


Siddiqui N, Kozlov G, D’Orso I, Trempe J-F, Gehring K (2003) Solution structure of the C-terminal domain from poly(A)-binding protein in Trypanosoma cruzi: a vegetal PABC domain. Protein Science 12 : 1925-1933. PMID 12930992


Trempe J-F, Denisov A, Gehring K (2003) Recoupling of residual dipolar couplings in single-domain polymer-stabilized liquid crystals undergoing magic-angle spinning. Journal of Magnetic Resonance 164: 329-337. PMID 14511601


Trempe J-F, Morin FG, Xia X, Marchessault RH, Gehring K (2002) Characterization of polyacrylamide-stabilized Pf1 phage liquid crystals for protein NMR spectroscopy. Journal of Biomolecular NMR 22: 83-87. PMID 11885983


Kozlov G, Siddiqui N, Coillet-Matillon S, Trempe J-F, Ekiel I, Sprules T, Gehring K (2002) Solution structure of the orphan PABC domain from Saccharomyces cerevisiae poly(A)-binding protein. Journal of Biological Chemistry 277 : 22822-22828. PMID 11940585  


Denisov AY, Noronha AM, Wilds CJ, Trempe J-F, Pon RT, Gehring K, Damha MJ (2001) Solution structure of an arabinonucleic acid (ANA)/RNA duplex in a chimeric hairpin: comparison with 2’-Fluoro-ANA/RNA and DNA/RNA Hybrids. Nucleic Acids Research 29: 4284-4293. PMID 11691916


Trempe J-F, Wilds CJ, Denisov AY, Pon RT, Damha MJ, Gehring K (2001) NMR solution structure of an oligonucleotide hairpin with a 2’F-ANA/RNA stem: implications for RNase H specificity toward DNA/RNA hybrid duplexes. Journal of the American Chemical Society 123: 4896-4903. PMID 11457316


Damha MJ#, Noronha AM, Wilds CJ, Trempe J-F, Denisov AY, Pon RT, Gehring K. (2001) Properties of arabinonucleic Acids (ANA & 2’F-ANA): implications for the design of antisense therapeutics that invoke RNase H cleavage of RNA. Nucleosides, Nucleotides & Nucleic Acids, 20 : 429-440. PMID 11563058

Kozlov G, Trempe J-F, Khaleghpour K, Kahvejian A, Ekiel I, Gehring K (2001) Structure and function of the C-terminal PABC domain of human poly(A)-binding protein. Proceedings of the National Academy of Sciences U.S.A. 98: 4409-4413. PMID 11287632

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